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microarray scanner  (Danaher Inc)


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    Structured Review

    Danaher Inc microarray scanner
    Microarray Scanner, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+assays/GenePix+Microarray+Systems/pm42772593-192-17-19
    Average 94 stars, based on 461 article reviews
    microarray scanner - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Dorsal root ganglion-derived exosomes deteriorate neuropathic pain by activating microglia via the microRNA-16-5p/HECTD1/HSP90 axis
    Article Snippet: .. The IL-6 and IL-1β levels in the cell culture supernatant were assessed using the Mouse IL-6 ELISA Kit (ab285330, Abcam) and Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (MLB00C, R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol. ..

    Article Title: Ovarian tumor cell-derived JAGGED2 promotes omental metastasis through stimulating the Notch signaling pathway in the mesothelial cells
    Article Snippet: .. Human IL-6 in the conditioned medium of cultured or cocultured cells were quantified in triplicate using an ELISA kit (Human ELISA kit, Abcam, USA, cat# ab-178013). .. The preclinical use, pharmacokinetics, and pharmacodynamics of MRK-003 (Merck Research Laboratories) have been well-reported previously [ ].

    Enzyme-linked Immunosorbent Assay:

    Article Title: Dorsal root ganglion-derived exosomes deteriorate neuropathic pain by activating microglia via the microRNA-16-5p/HECTD1/HSP90 axis
    Article Snippet: .. The IL-6 and IL-1β levels in the cell culture supernatant were assessed using the Mouse IL-6 ELISA Kit (ab285330, Abcam) and Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (MLB00C, R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol. ..

    Article Title: LncRNA 1700020I14Rik promotes AKR1B10 expression and activates Erk pathway to induce hepatocyte damage in alcoholic hepatitis
    Article Snippet: .. Serum levels of ALT, AST, TNF-α, and IL-6 were detected in line with the kit instructions of the mouse ALT ELISA kit (ab282882, Abcam), mouse AST ELISA kit (ab263882, Abcam), mouse TNF-α ELISA kit (ab208348, Abcam), and mouse IL-6 ELISA Kit (ab222503, Abcam). .. The 4% formalin-fixed liver tissues were parraffin-embedded and cut into 5 μm serial sections, which were stained with the hematoxylin-eosin (H&E) staining kit (C0105S, Beyotime) [ ].

    Article Title: Microglial apolipoprotein E particles contribute to neuronal senescence and synaptotoxicity
    Article Snippet: .. Mouse Apolipoprotein E ELISA Kit , Abcam , Cat#ab215086. .. Lipid removal agent (LRA) , Millipore Sigma , Cat#13358-U.

    Article Title: Qingre Xingyu recipe exerts inhibiting effects on ulcerative colitis development by inhibiting TNFα/NLRP3/Caspase-1/IL-1β pathway and macrophage M1 polarization
    Article Snippet: .. The kits used for supernatant isolation of mouse colon explants were as follows: mouse interleukin-6 (IL-6) ELISA kit (ab100713, Abcam), mouse interleukin-8 (IL-8) ELISA kit (ml063162, LiankeBio, Hangzhou, China), mouse interleukin-1β (IL-1β) ELISA kit (ab197742, Abcam) and mouse TNFα ELISA kit (ab208348, Abcam). .. The kits used for supernatant isolation of Caco-2 cells were as follows: human IL-6 ELISA kit (ab178013, Abcam) and human IL-8 ELISA kit (ab214030, Abcam).

    Article Title: Ovarian tumor cell-derived JAGGED2 promotes omental metastasis through stimulating the Notch signaling pathway in the mesothelial cells
    Article Snippet: .. Human IL-6 in the conditioned medium of cultured or cocultured cells were quantified in triplicate using an ELISA kit (Human ELISA kit, Abcam, USA, cat# ab-178013). .. The preclinical use, pharmacokinetics, and pharmacodynamics of MRK-003 (Merck Research Laboratories) have been well-reported previously [ ].

    Article Title: Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-human-IgG-Cy3 Sigma Aldrich RRID: AB_258804 Cat#: C2571-1ML PGT128, PGT121, PGT151, PGT130, PGT123 (Walker et al., 2011) N/A VRC01 (Zhou et al., 2010) N/A F425-b4e8 (Cavacini et al., 2003) N/A 2G12 (Trkola et al., 1996) N/A .. PCDN76 22-A, PCDN76 22-C, PCDN76 38-A or PCDN76 38-B (Macleod et al., 2016) N/A Goat a-human-Alexa Fluor 488 Jackson ImmunoResearch RRID:AB_2337833 Cat#:109-545-008 Protein G-HRP Abcam Cat#: ab7460 Goat-anti-human IgG H+L 800CW Li-Cor Cat#: 926-32232 Goat-anti-human-Fc-AP Jackson RRID: AB_2337608 Cat#:109-055-098 Biological samples International AIDS vaccine initiative IAVI Protocol C cohort (Landais et al., 2016) N/A Chemicals, peptides, and recombinant proteins Biotinylated Phaseolus vulgaris Leucoagglutinin (PHA-L) Vector laboratories Cat#: B-1115 Biotinylated Concanavalin A (Con A) Vector laboratories Cat#: B-1005 DAPI Sigma Aldrich Cat#: D9542-1MG Rhodamine phalloidin Life technologies Cat#: R415 Gp120 (JR-CSF) This manuscript N/A Critical commercial assays SignalFireTM ECL Reagent Cell Signaling Technology Cat#: 6883P3 Human Anti-Schistosoma mansoni IgG ELISA Kit Abcam Cat#: ab108769 Experimental models: Organisms/strains Cercariae were shed from Biomphalaria glabrata snails infected with Schistosoma mansoni (strain NMRI) NIAID Schistosomiasis Resource Center N/A Software and algorithms Prism Graphpad https://www.graphpad.com/ scientific-software/prism/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC https://www.pymol.org/ GenePix Pro 6.0 software Molecular Devices N/A Fiji Image J N/A Other VictorTM X3 multilabel reader Perkin Elmer N/A Agilent G2565BA microarray scanner system Agilent Technologies, Santa Clara, USA N/A Nikon A1 inverted confocal microscope Nikon Centre, KCL N/A Li-Cor Odyssey XF Imaging System Li-Cor N/A ProScanArray Express Perkin Elmer, Shelton, USA N/A ImageQuant Detector GE Life Science N/A e1 Cell Reports 38, 110611, March 29, 2022 ..

    AST Assay:

    Article Title: LncRNA 1700020I14Rik promotes AKR1B10 expression and activates Erk pathway to induce hepatocyte damage in alcoholic hepatitis
    Article Snippet: .. Serum levels of ALT, AST, TNF-α, and IL-6 were detected in line with the kit instructions of the mouse ALT ELISA kit (ab282882, Abcam), mouse AST ELISA kit (ab263882, Abcam), mouse TNF-α ELISA kit (ab208348, Abcam), and mouse IL-6 ELISA Kit (ab222503, Abcam). .. The 4% formalin-fixed liver tissues were parraffin-embedded and cut into 5 μm serial sections, which were stained with the hematoxylin-eosin (H&E) staining kit (C0105S, Beyotime) [ ].

    Recombinant:

    Article Title: Neutrophil ALDH2 is a new therapeutic target for the effective treatment of sepsis-induced ARDS.
    Article Snippet: Acetaldehyde dehydrogenase 2 (ALDH2) mutations are commonly found in a subgroup of the Asian population.. However, the role of ALDH2 in septic acute respiratory distress syndrome (ARDS) remains unknown.. Here, we showed that human subjects carrying the ALDH2 mutation were highly susceptible to developing septic ARDS.

    Article Title: Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-human-IgG-Cy3 Sigma Aldrich RRID: AB_258804 Cat#: C2571-1ML PGT128, PGT121, PGT151, PGT130, PGT123 (Walker et al., 2011) N/A VRC01 (Zhou et al., 2010) N/A F425-b4e8 (Cavacini et al., 2003) N/A 2G12 (Trkola et al., 1996) N/A .. PCDN76 22-A, PCDN76 22-C, PCDN76 38-A or PCDN76 38-B (Macleod et al., 2016) N/A Goat a-human-Alexa Fluor 488 Jackson ImmunoResearch RRID:AB_2337833 Cat#:109-545-008 Protein G-HRP Abcam Cat#: ab7460 Goat-anti-human IgG H+L 800CW Li-Cor Cat#: 926-32232 Goat-anti-human-Fc-AP Jackson RRID: AB_2337608 Cat#:109-055-098 Biological samples International AIDS vaccine initiative IAVI Protocol C cohort (Landais et al., 2016) N/A Chemicals, peptides, and recombinant proteins Biotinylated Phaseolus vulgaris Leucoagglutinin (PHA-L) Vector laboratories Cat#: B-1115 Biotinylated Concanavalin A (Con A) Vector laboratories Cat#: B-1005 DAPI Sigma Aldrich Cat#: D9542-1MG Rhodamine phalloidin Life technologies Cat#: R415 Gp120 (JR-CSF) This manuscript N/A Critical commercial assays SignalFireTM ECL Reagent Cell Signaling Technology Cat#: 6883P3 Human Anti-Schistosoma mansoni IgG ELISA Kit Abcam Cat#: ab108769 Experimental models: Organisms/strains Cercariae were shed from Biomphalaria glabrata snails infected with Schistosoma mansoni (strain NMRI) NIAID Schistosomiasis Resource Center N/A Software and algorithms Prism Graphpad https://www.graphpad.com/ scientific-software/prism/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC https://www.pymol.org/ GenePix Pro 6.0 software Molecular Devices N/A Fiji Image J N/A Other VictorTM X3 multilabel reader Perkin Elmer N/A Agilent G2565BA microarray scanner system Agilent Technologies, Santa Clara, USA N/A Nikon A1 inverted confocal microscope Nikon Centre, KCL N/A Li-Cor Odyssey XF Imaging System Li-Cor N/A ProScanArray Express Perkin Elmer, Shelton, USA N/A ImageQuant Detector GE Life Science N/A e1 Cell Reports 38, 110611, March 29, 2022 ..

    Isolation:

    Article Title: Qingre Xingyu recipe exerts inhibiting effects on ulcerative colitis development by inhibiting TNFα/NLRP3/Caspase-1/IL-1β pathway and macrophage M1 polarization
    Article Snippet: .. The kits used for supernatant isolation of mouse colon explants were as follows: mouse interleukin-6 (IL-6) ELISA kit (ab100713, Abcam), mouse interleukin-8 (IL-8) ELISA kit (ml063162, LiankeBio, Hangzhou, China), mouse interleukin-1β (IL-1β) ELISA kit (ab197742, Abcam) and mouse TNFα ELISA kit (ab208348, Abcam). .. The kits used for supernatant isolation of Caco-2 cells were as follows: human IL-6 ELISA kit (ab178013, Abcam) and human IL-8 ELISA kit (ab214030, Abcam).

    Infection:

    Article Title: Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-human-IgG-Cy3 Sigma Aldrich RRID: AB_258804 Cat#: C2571-1ML PGT128, PGT121, PGT151, PGT130, PGT123 (Walker et al., 2011) N/A VRC01 (Zhou et al., 2010) N/A F425-b4e8 (Cavacini et al., 2003) N/A 2G12 (Trkola et al., 1996) N/A .. PCDN76 22-A, PCDN76 22-C, PCDN76 38-A or PCDN76 38-B (Macleod et al., 2016) N/A Goat a-human-Alexa Fluor 488 Jackson ImmunoResearch RRID:AB_2337833 Cat#:109-545-008 Protein G-HRP Abcam Cat#: ab7460 Goat-anti-human IgG H+L 800CW Li-Cor Cat#: 926-32232 Goat-anti-human-Fc-AP Jackson RRID: AB_2337608 Cat#:109-055-098 Biological samples International AIDS vaccine initiative IAVI Protocol C cohort (Landais et al., 2016) N/A Chemicals, peptides, and recombinant proteins Biotinylated Phaseolus vulgaris Leucoagglutinin (PHA-L) Vector laboratories Cat#: B-1115 Biotinylated Concanavalin A (Con A) Vector laboratories Cat#: B-1005 DAPI Sigma Aldrich Cat#: D9542-1MG Rhodamine phalloidin Life technologies Cat#: R415 Gp120 (JR-CSF) This manuscript N/A Critical commercial assays SignalFireTM ECL Reagent Cell Signaling Technology Cat#: 6883P3 Human Anti-Schistosoma mansoni IgG ELISA Kit Abcam Cat#: ab108769 Experimental models: Organisms/strains Cercariae were shed from Biomphalaria glabrata snails infected with Schistosoma mansoni (strain NMRI) NIAID Schistosomiasis Resource Center N/A Software and algorithms Prism Graphpad https://www.graphpad.com/ scientific-software/prism/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC https://www.pymol.org/ GenePix Pro 6.0 software Molecular Devices N/A Fiji Image J N/A Other VictorTM X3 multilabel reader Perkin Elmer N/A Agilent G2565BA microarray scanner system Agilent Technologies, Santa Clara, USA N/A Nikon A1 inverted confocal microscope Nikon Centre, KCL N/A Li-Cor Odyssey XF Imaging System Li-Cor N/A ProScanArray Express Perkin Elmer, Shelton, USA N/A ImageQuant Detector GE Life Science N/A e1 Cell Reports 38, 110611, March 29, 2022 ..

    Software:

    Article Title: Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-human-IgG-Cy3 Sigma Aldrich RRID: AB_258804 Cat#: C2571-1ML PGT128, PGT121, PGT151, PGT130, PGT123 (Walker et al., 2011) N/A VRC01 (Zhou et al., 2010) N/A F425-b4e8 (Cavacini et al., 2003) N/A 2G12 (Trkola et al., 1996) N/A .. PCDN76 22-A, PCDN76 22-C, PCDN76 38-A or PCDN76 38-B (Macleod et al., 2016) N/A Goat a-human-Alexa Fluor 488 Jackson ImmunoResearch RRID:AB_2337833 Cat#:109-545-008 Protein G-HRP Abcam Cat#: ab7460 Goat-anti-human IgG H+L 800CW Li-Cor Cat#: 926-32232 Goat-anti-human-Fc-AP Jackson RRID: AB_2337608 Cat#:109-055-098 Biological samples International AIDS vaccine initiative IAVI Protocol C cohort (Landais et al., 2016) N/A Chemicals, peptides, and recombinant proteins Biotinylated Phaseolus vulgaris Leucoagglutinin (PHA-L) Vector laboratories Cat#: B-1115 Biotinylated Concanavalin A (Con A) Vector laboratories Cat#: B-1005 DAPI Sigma Aldrich Cat#: D9542-1MG Rhodamine phalloidin Life technologies Cat#: R415 Gp120 (JR-CSF) This manuscript N/A Critical commercial assays SignalFireTM ECL Reagent Cell Signaling Technology Cat#: 6883P3 Human Anti-Schistosoma mansoni IgG ELISA Kit Abcam Cat#: ab108769 Experimental models: Organisms/strains Cercariae were shed from Biomphalaria glabrata snails infected with Schistosoma mansoni (strain NMRI) NIAID Schistosomiasis Resource Center N/A Software and algorithms Prism Graphpad https://www.graphpad.com/ scientific-software/prism/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC https://www.pymol.org/ GenePix Pro 6.0 software Molecular Devices N/A Fiji Image J N/A Other VictorTM X3 multilabel reader Perkin Elmer N/A Agilent G2565BA microarray scanner system Agilent Technologies, Santa Clara, USA N/A Nikon A1 inverted confocal microscope Nikon Centre, KCL N/A Li-Cor Odyssey XF Imaging System Li-Cor N/A ProScanArray Express Perkin Elmer, Shelton, USA N/A ImageQuant Detector GE Life Science N/A e1 Cell Reports 38, 110611, March 29, 2022 ..

    Microarray:

    Article Title: Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-human-IgG-Cy3 Sigma Aldrich RRID: AB_258804 Cat#: C2571-1ML PGT128, PGT121, PGT151, PGT130, PGT123 (Walker et al., 2011) N/A VRC01 (Zhou et al., 2010) N/A F425-b4e8 (Cavacini et al., 2003) N/A 2G12 (Trkola et al., 1996) N/A .. PCDN76 22-A, PCDN76 22-C, PCDN76 38-A or PCDN76 38-B (Macleod et al., 2016) N/A Goat a-human-Alexa Fluor 488 Jackson ImmunoResearch RRID:AB_2337833 Cat#:109-545-008 Protein G-HRP Abcam Cat#: ab7460 Goat-anti-human IgG H+L 800CW Li-Cor Cat#: 926-32232 Goat-anti-human-Fc-AP Jackson RRID: AB_2337608 Cat#:109-055-098 Biological samples International AIDS vaccine initiative IAVI Protocol C cohort (Landais et al., 2016) N/A Chemicals, peptides, and recombinant proteins Biotinylated Phaseolus vulgaris Leucoagglutinin (PHA-L) Vector laboratories Cat#: B-1115 Biotinylated Concanavalin A (Con A) Vector laboratories Cat#: B-1005 DAPI Sigma Aldrich Cat#: D9542-1MG Rhodamine phalloidin Life technologies Cat#: R415 Gp120 (JR-CSF) This manuscript N/A Critical commercial assays SignalFireTM ECL Reagent Cell Signaling Technology Cat#: 6883P3 Human Anti-Schistosoma mansoni IgG ELISA Kit Abcam Cat#: ab108769 Experimental models: Organisms/strains Cercariae were shed from Biomphalaria glabrata snails infected with Schistosoma mansoni (strain NMRI) NIAID Schistosomiasis Resource Center N/A Software and algorithms Prism Graphpad https://www.graphpad.com/ scientific-software/prism/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC https://www.pymol.org/ GenePix Pro 6.0 software Molecular Devices N/A Fiji Image J N/A Other VictorTM X3 multilabel reader Perkin Elmer N/A Agilent G2565BA microarray scanner system Agilent Technologies, Santa Clara, USA N/A Nikon A1 inverted confocal microscope Nikon Centre, KCL N/A Li-Cor Odyssey XF Imaging System Li-Cor N/A ProScanArray Express Perkin Elmer, Shelton, USA N/A ImageQuant Detector GE Life Science N/A e1 Cell Reports 38, 110611, March 29, 2022 ..

    Microscopy:

    Article Title: Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-human-IgG-Cy3 Sigma Aldrich RRID: AB_258804 Cat#: C2571-1ML PGT128, PGT121, PGT151, PGT130, PGT123 (Walker et al., 2011) N/A VRC01 (Zhou et al., 2010) N/A F425-b4e8 (Cavacini et al., 2003) N/A 2G12 (Trkola et al., 1996) N/A .. PCDN76 22-A, PCDN76 22-C, PCDN76 38-A or PCDN76 38-B (Macleod et al., 2016) N/A Goat a-human-Alexa Fluor 488 Jackson ImmunoResearch RRID:AB_2337833 Cat#:109-545-008 Protein G-HRP Abcam Cat#: ab7460 Goat-anti-human IgG H+L 800CW Li-Cor Cat#: 926-32232 Goat-anti-human-Fc-AP Jackson RRID: AB_2337608 Cat#:109-055-098 Biological samples International AIDS vaccine initiative IAVI Protocol C cohort (Landais et al., 2016) N/A Chemicals, peptides, and recombinant proteins Biotinylated Phaseolus vulgaris Leucoagglutinin (PHA-L) Vector laboratories Cat#: B-1115 Biotinylated Concanavalin A (Con A) Vector laboratories Cat#: B-1005 DAPI Sigma Aldrich Cat#: D9542-1MG Rhodamine phalloidin Life technologies Cat#: R415 Gp120 (JR-CSF) This manuscript N/A Critical commercial assays SignalFireTM ECL Reagent Cell Signaling Technology Cat#: 6883P3 Human Anti-Schistosoma mansoni IgG ELISA Kit Abcam Cat#: ab108769 Experimental models: Organisms/strains Cercariae were shed from Biomphalaria glabrata snails infected with Schistosoma mansoni (strain NMRI) NIAID Schistosomiasis Resource Center N/A Software and algorithms Prism Graphpad https://www.graphpad.com/ scientific-software/prism/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC https://www.pymol.org/ GenePix Pro 6.0 software Molecular Devices N/A Fiji Image J N/A Other VictorTM X3 multilabel reader Perkin Elmer N/A Agilent G2565BA microarray scanner system Agilent Technologies, Santa Clara, USA N/A Nikon A1 inverted confocal microscope Nikon Centre, KCL N/A Li-Cor Odyssey XF Imaging System Li-Cor N/A ProScanArray Express Perkin Elmer, Shelton, USA N/A ImageQuant Detector GE Life Science N/A e1 Cell Reports 38, 110611, March 29, 2022 ..

    Imaging:

    Article Title: Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-human-IgG-Cy3 Sigma Aldrich RRID: AB_258804 Cat#: C2571-1ML PGT128, PGT121, PGT151, PGT130, PGT123 (Walker et al., 2011) N/A VRC01 (Zhou et al., 2010) N/A F425-b4e8 (Cavacini et al., 2003) N/A 2G12 (Trkola et al., 1996) N/A .. PCDN76 22-A, PCDN76 22-C, PCDN76 38-A or PCDN76 38-B (Macleod et al., 2016) N/A Goat a-human-Alexa Fluor 488 Jackson ImmunoResearch RRID:AB_2337833 Cat#:109-545-008 Protein G-HRP Abcam Cat#: ab7460 Goat-anti-human IgG H+L 800CW Li-Cor Cat#: 926-32232 Goat-anti-human-Fc-AP Jackson RRID: AB_2337608 Cat#:109-055-098 Biological samples International AIDS vaccine initiative IAVI Protocol C cohort (Landais et al., 2016) N/A Chemicals, peptides, and recombinant proteins Biotinylated Phaseolus vulgaris Leucoagglutinin (PHA-L) Vector laboratories Cat#: B-1115 Biotinylated Concanavalin A (Con A) Vector laboratories Cat#: B-1005 DAPI Sigma Aldrich Cat#: D9542-1MG Rhodamine phalloidin Life technologies Cat#: R415 Gp120 (JR-CSF) This manuscript N/A Critical commercial assays SignalFireTM ECL Reagent Cell Signaling Technology Cat#: 6883P3 Human Anti-Schistosoma mansoni IgG ELISA Kit Abcam Cat#: ab108769 Experimental models: Organisms/strains Cercariae were shed from Biomphalaria glabrata snails infected with Schistosoma mansoni (strain NMRI) NIAID Schistosomiasis Resource Center N/A Software and algorithms Prism Graphpad https://www.graphpad.com/ scientific-software/prism/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC https://www.pymol.org/ GenePix Pro 6.0 software Molecular Devices N/A Fiji Image J N/A Other VictorTM X3 multilabel reader Perkin Elmer N/A Agilent G2565BA microarray scanner system Agilent Technologies, Santa Clara, USA N/A Nikon A1 inverted confocal microscope Nikon Centre, KCL N/A Li-Cor Odyssey XF Imaging System Li-Cor N/A ProScanArray Express Perkin Elmer, Shelton, USA N/A ImageQuant Detector GE Life Science N/A e1 Cell Reports 38, 110611, March 29, 2022 ..



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    Innopsys Inc innoscan 710 high resolution microarray scanner
    BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell <t>Microarray</t> Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.
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    BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell Microarray Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.

    Journal: iScience

    Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity

    doi: 10.1016/j.isci.2026.116022

    Figure Lengend Snippet: BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell Microarray Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.

    Article Snippet: The Retrogenix cell microarray was performed by Charles River Laboratories and included 6,105 full-length human proteins (plasma membrane proteins, secreted or a cell surface-tethered secreted proteins) and 400 human heterodimers.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, Microarray, Flow Cytometry, Transfection, Positive Control